ABSTRACT
Objective To investigate the prevalence of patients with hypertension complicated with heart failure in Shenyang, and to understand population characteristics and risk factors. Methods From January 2018 to December 2018, a large-scale random sampling survey was conducted in various districts and counties in Shenyang, Liaoning, to investigate the study subjects’ physical indicators and laboratory examination indicators through family visits using questionnaires. Results A total of 32 257 people were randomly sampled in Shenyang, with 32 140 valid questionnaires. The questionnaire validity rate was 99.63%. The prevalence of hypertension combined with heart failure was 9.14%, and the disease awareness rate of the patients was 41.46%. There were significant differences in the incidence rates of the patients with different gender, age, residence, occupation, BMI, abdominal obesity, dyslipidemia, diabetes and family history of the disease (P<0.05), among which age, BMI, abdominal obesity, dyslipidemia, diabetes and family history were risk factors for hypertension complicated with heart failure. There were statistically significant differences in disease awareness rates among people with different gender, age, residence, education level, occupation, BMI, abdominal obesity, dyslipidemia, diabetes and family history (P<0.05). The disease awareness rate was mainly affected by gender, age, residence, education level and occupation. Conclusion The prevalence of hypertension complicated with heart failure in Shenyang is related to a variety of factors, among which age, BMI, abdominal obesity, dyslipidemia, diabetes and family history of the disease were risk factors. The disease awareness rate of patients is also low. Therefore, reasonable and effective measures should be taken to reduce the incidence of the disease.
ABSTRACT
Objective We conduct this research in order to understand the satisfaction rate of vaccination outpatient service and awareness rate of vaccination knowledge among parents ,and to provide scientific basis for strengthening immune programming . Methods We used unified and structured questionnaire suggested by Regulations for Management of Vaccination Units in Sichuan Province .A total number of 116 vaccination outpatient sections were included in our research and in each outpatient section ,30 par-ents were investigated .Results In children′s parents ,the overall satisfaction rate of vaccination outpatient service was 98 .07% , with the highest satisfaction rate(96 .41% ) in technical skills of vaccination staffs ,and lowest satisfaction rate(94 .34% ) in the en-vironment of vaccination outpatient section .The overall awareness rate of vaccination knowledge was 88 .02% ,with the highest awareness rate(100 .00% ) in the question “If it is necessary to take vaccines after child′s birth” ,and lowest awareness rate (67 .93% ) in the question“Which are the three kinds of national immunization vaccines” .Conclusion In Chengdu ,the satisfaction rate of vaccination service is quite high among parents and most of the parents understand the necessity of taking vaccines ,but the awareness rates of questions related to vaccines are low .Therefore ,it is necessary to continue pushing standardized construction of vaccination outpatient service and strengthen health education about immune programming .
ABSTRACT
The cDNA of cattle Ghrelin gene was amplified from abomasum fundic gland mRNA of Qinchuan Cattle by RT-PCR. PCR product was cloned into the T vector pGM-T to construct pGh-T1 for sequencing. Then the cDNA was subcloned into the prokaryotic expressing plasmid vector pET32a (+) and transformed into host Escherichia coli strain BL21 (DE3) for expression. The expression of pGh-32 mature Ghrelin protein was induced by IPTG and was identified by SDS-PAGE. The expression product was observed with soluble protein and inclusion body. Western blotting showed that the recombinant protein was recognized by his-antibody specifically. The protein was purified by Ni-NTA column and was used to inject rabbits to obtain polyclona antibody. ELISA result showed that the antibody titer was 1:12 800. The immunohistochemistry test between the hypothalamus arcuate nucleus and the antibody showed that fusion protein had biological activity. This will provide a basis for further study on the biological function of Ghrelin protein to growth and development and fat deposition of cattle.